Part:BBa_K4117002:Design
CYP2C9
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 127
Illegal PstI site found at 1357 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 127
Illegal PstI site found at 1357
Illegal NotI site found at 284 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1386
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 127
Illegal PstI site found at 1357 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 127
Illegal PstI site found at 1357 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since this subunit has been proved to be expressed successfully, we did not re-engineer this protein gene.
Source
We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from NCBI:P33261.4
References
[1]Gasse A, Vennemann M, Köhler H, Schürenkamp J. Toxicogenetic analysis of Δ9-THC-metabolizing enzymes. Int J Legal Med. 2020 Nov;134(6):2095-2103. doi: 10.1007/s00414-020-02380-3. Epub 2020 Jul 25. PMID: 32712703; PMCID: PMC7578149.
[2]Watanabe K, Yamaori S, Funahashi T, Kimura T, Yamamoto I. Cytochrome P450 enzymes involved in the metabolism of tetrahydrocannabinols and cannabinol by human hepatic microsomes. Life Sci. 2007 Mar 20;80(15):1415-9. doi: 10.1016/j.lfs.2006.12.032. Epub 2007 Jan 17. PMID: 17303175.
[3]Doohan, P.T., Oldfield, L.D., Arnold, J.C. et al. Cannabinoid Interactions with Cytochrome P450 Drug Metabolism: a Full-Spectrum Characterization. AAPS J 23, 91 (2021). https://doi.org/10.1208/s12248-021-00616-7
[4]Patilea-Vrana GI, Anoshchenko O, Unadkat JD. Hepatic Enzymes Relevant to the Disposition of (-)-∆9-Tetrahydrocannabinol (THC) and Its Psychoactive Metabolite, 11-OH-THC. Drug Metab Dispos. 2019 Mar;47(3):249-256. doi: 10.1124/dmd.118.085548. Epub 2018 Dec 19. PMID: 30567877; PMCID: PMC6374540.